SDS Page:Article Title: Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE , transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713-035-147); mouse anti-BLVRA (1:1000; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205-032-176); mouse anti-BlvrB (Santa Cruz; Dallas, TX cat#sc-373692) followed by goat anti-mouse (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); rabbit anti-PF4 (1:500; Abcam; Cambridge, UK cat#ab129183) followed by donkey anti-rabbit (1:5000; Millipore Sigma; Burlington, MA cat#MANA9340); rabbit anti-CD61 (1:1000; Proteintech; Rosement, IL cat#18309-1-AP) followed by donkey anti-rabbit (1:5000, Millipore Sigma, Burlington, MA cat#NA9340); mouse anti-actin antibody (1:1000; Millipore Sigma; Burlington, MA cat#MAB1501R) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); mouse anti-GAPDH antibody (1:1000; Invitrogen; Carlsbad, CA cat#MA5-15738) followed by goat anti-mouse antibody (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072). .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Article Title: Biliverdin reductase B as a new target in breast cancer.
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [37], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/ Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Article Title: Biliverdin reductase B as a new target in breast cancer
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [ ], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035-147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Immunodetection:Article Title: Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE , transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713-035-147); mouse anti-BLVRA (1:1000; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205-032-176); mouse anti-BlvrB (Santa Cruz; Dallas, TX cat#sc-373692) followed by goat anti-mouse (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); rabbit anti-PF4 (1:500; Abcam; Cambridge, UK cat#ab129183) followed by donkey anti-rabbit (1:5000; Millipore Sigma; Burlington, MA cat#MANA9340); rabbit anti-CD61 (1:1000; Proteintech; Rosement, IL cat#18309-1-AP) followed by donkey anti-rabbit (1:5000, Millipore Sigma, Burlington, MA cat#NA9340); mouse anti-actin antibody (1:1000; Millipore Sigma; Burlington, MA cat#MAB1501R) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); mouse anti-GAPDH antibody (1:1000; Invitrogen; Carlsbad, CA cat#MA5-15738) followed by goat anti-mouse antibody (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072). .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Article Title: Biliverdin reductase B as a new target in breast cancer.
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [37], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/ Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Article Title: Small molecule BLVRB redox inhibitor promotes megakaryocytopoiesis and stress thrombopoiesis in vivo.
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE7, transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch;West Grove, PA; cat#713-035-147);mouse anti-BLVRA (1:1000; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205-032-176); mouse anti-BlvrB (Santa Cruz; Dallas, TX cat#sc-373692) followed by goat anti-mouse (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); rabbit anti-PF4 (1:500; Abcam; Cambridge, UK cat#ab129183) followed by donkey anti-rabbit (1:5000; Millipore Sigma; Burlington, MA cat#MANA9340); rabbit anti-CD61 (1:1000; Proteintech; Rosement, IL cat#18309-1-AP) followed by donkey antirabbit (1:5000, Millipore Sigma, Burlington, MA cat#NA9340); mouse anti-actin antibody (1:1000; Millipore Sigma; Burlington, MA cat#MAB1501R) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072); mouse anti- Nature Communications | (2025) 16:3480 14 GAPDH antibody (1:1000; Invitrogen; Carlsbad, CA cat#MA5-15738) followed by goat anti-mouse antibody (1:5000; Jackson ImmunoResearch; West Grove, PA cat#115-036-072). .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Article Title: Biliverdin reductase B as a new target in breast cancer
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [ ], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035-147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Marker:Article Title: Biliverdin reductase B as a new target in breast cancer.
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [37], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/ Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
Article Title: Biliverdin reductase B as a new target in breast cancer
Article Snippet: .. Protein lysates were size-fractionated by SDS-PAGE [ ], transferred to nitrocellulose membranes, and immunodetection was completed using sheep anti-BLVRB antibody (1:1000; R&D Systems; Minneapolis, MN cat#AF6568) followed by donkey anti-sheep antibody (1:2000; Jackson ImmunoResearch; West Grove, PA; cat#713–035-147); mouse anti-BLVRA (1:500; Santa Cruz; Dallas, TX cat#sc-393385) followed by goat anti-mouse antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#115–585-003); goat anti-Transferrin Receptor (CD71; R & D Systems, Minneapolis, MN catAF2474) followed by mouse anti-goat antibody (1:2000; Jackson ImmunoResearch; West Grove, PA cat#205–032-176); mouse anti-HNE antibody (1:1,000; Thermo Fisher Scientific, cat#MA5-27,570) followed by sheep anti-mouse antibody (1:10,000 dilution), mouse anti-heme oxygenase I (1:1000; Novus Biologicals; Centennial, CO cat#NBP1-97,507) followed by sheep anti-mouse antibody (1:10,000); mouse anti-heme oxygenase 2 (1:100; Santa Cruz; Dallas, TX cat#sc-17786) followed by sheep anti-mouse antibody (1:10,000) mouse anti-actin antibody (1:1,000; Thermo Fisher Scientific; Waltham, MA cat#MA1-744) followed by sheep anti-mouse antibody (1:10,000; GE Healthcare; Chicago, IL cat#NA9310) Hsc70 (#sc-71270) Hsp90 (#sc-69703) (all from Santa Cruz Biotechnology); NQO1 (#62,262), PERK, BIP, CHOP (ER Stress Antibody Sampler Kit #9956), PARP (#9542), HER2, pHER2(Y1221/Y1222) (HER/ErbB Family Antibody Sampler Kit #8339), Pan-R-Tyr (Phospho-Tyrosine (P-Tyr-1000) MultiMab® Rabbit mAb mix #8954), EE1A, caleolin, clathrin, RAB5A, RAB7 (Endosomal Marker Antibody Sampler Kit #12,666) all from Cell Signaling. .. Immunodetection was completed by enhanced chemiluminescence using LuminataTM Forte Western HRP substrate (Millipore Sigma; Burlington, MA) and visualized using a C-DiGit® blot scanner (LI-COR; Lincoln, NE).
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